Apoptosis Influencing Activity of Latex of Euphorbia antiquoram Linn in Mice
Hamsa D, Sumathi S, Kavithaa K, Sowmini CM, Padma PR
Programmed cell death (PCD) describes a physiological and pathological process of cell deletion that plays an important role in maintaining tissue homeostasis. It is a highly regulated cellular suicide process essential for growth and survival in all eukaryotes. Mouse spleen cells have been successfully used to analyze mechanisms of cytotoxicity for a variety of anticancer drugs. In the present study we analyzed the apoptosis influencing effect of latex of Euphorbia antiquoram using mice spleenocytes revealed by staining techniques such as AO and EtBr, PI, EtBr and DAPI. The extent of DNA damage was assessed on exposure to etoposide with or without latex milk of Euphorbia antiquoram by agarose gel electrophoresis. The present study revealed that the latex was able to induce apoptosis as evidenced by staining techniques Etoposide caused a maximal DNA damage in spleen cells, which are non cancerous and untransformed cells. This effect was effectively counteracted by the latex milk of Euphorbia antiquorum. These findings potentiate the use of latex milk of Euphorbia antiquorum in combination therapy when this extract can overcome the toxic side effect of chemotherapeutic drug, because it is safe to normal cells.
Apoptosis, Cytotoxicity, DNA Damage, Euphorbia Antiqoram
Cite This Article
Hamsa D, Sumathi S, Kavithaa K, Sowmini CM, Padma PR, Apoptosis Influencing Activity of Latex of Euphorbia antiquoram Linn in Mice, International Journal for Pharmaceutical Research Scholars, 2013, 2(4), 238-243.