Fluorescein Derivative: Synthesis and Significance of 2-(7,8-Dihydro-10-Methyl-3,8-Dioxo-3H-Benzo[B]Xanthen-12-Yl) Benzoic Acid
Manisha M. Kodape, Nandkishor D. Gawhale, Pritee N. Chunarkar, Digamber M. Mutkule
Many fluorescein derivatives have functional groups that are suitable for reaction with other molecules, and can therefore serve as labels in a variety of analytical applications ranging from probing cell functions to monitoring the level of drugs in human fluids via immunoassays. We have tried to synthesize new derivative of Fluorescein by adding substituent on the fluorescein molecule. In present methodology Fluorescein was synthesized from resorcinol and Phthalic anhydride. From Fluorescein by adding ethyl acetoacetate and sulphuric acid, in ethanol and water under microwave irradiation 2-(7,8-dihydro-10-methyl-3,8-dioxo-3H-benzo[b]xanthen-12-yl)benzoic acid (P) was synthesized. This fluorescein derivative shows Minimum Inhibition Growth (MIC) against Proteus mirabilis pathogen.
Fluorescein, Resorcinol, Phallic Anhydride, Ethyl Acetoacetate
Cite This Article
Manisha M. Kodape, Nandkishor D. Gawhale, Pritee N. Chunarkar, Digamber M. Mutkule, Fluorescein Derivative: Synthesis and Significance of 2-(7,8-Dihydro-10-Methyl-3,8-Dioxo-3H-Benzo[B]Xanthen-12-Yl) Benzoic Acid, International Journal for Pharmaceutical Research Scholars, 2013, 2(4), 352-355.