Phylogenetic Analysis of Excoecaria Agallocha Using 18S Nuclear Ribosomal Gene

Phylogenetic Analysis of Excoecaria Agallocha Using 18S Nuclear Ribosomal Gene


Poonam Salve, Vishwesh R. Potkar, Pratima S. Jadhav


Abstract

Mangroves are woody plants which form the dominant variation in tidal, saline wetlands along tropical and subtropical coasts. Excoecaria agallocha is a mangrove species in the genus Excoecaria of the family Euphorbiaceae. 18S rRNA gene is one of the most important molecular markers, used in diverse applications such as molecular phylogenetic analyses and biodiversity screening. In the present study 18S gene of Excoecaria agallocha was amplified and sequenced. 18S gene was found to be 1659 base pairs (bp) in length where as GC% found 50.8. The number of nucleotide found to be 401 A's, 378 C's, 464 G's, 416 T's, and 0 N's. Phylogenetic tree infer that Excoecaria agallocha is closely related to Rhizophora stylosa and Xylocarpus granatum. Along with these two species Excoecaria agallocha forming monophyletic group with other closely related species such as Cerbera manghas, Kandelia candel, Bruguiera gymnorhiza and Nypa fruticans.


Keywords

Excoecaria agallocha, Phylogenetic, rRNA gene


Cite This Article

Poonam Salve, Vishwesh R. Potkar, Pratima S. Jadhav, Phylogenetic Analysis of Excoecaria Agallocha Using 18S Nuclear Ribosomal Gene, International Journal for Pharmaceutical Research Scholars, 2013, 2(4), 503-506.

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