Pattern of Diversity Using Molecular Marker (nrITS): An Example from Acanthaceae and Euphorbiaceae
Vishwesh R. Potkar, Satish N. Pardeshi, Pratima S. Jadhav
Molecular sequence data was used from the nuclear ribosomal internal transcribed spacers (ITS) to study phylogenetic relationship within Acanthaceae and Euphorbiaceae. 8 Species belonging to family Acanthaceae and 2 species belonging to family Euphorbiaceae were collected from different locations of Mumbai for this present study. The ribosomal internal transcribed spacers (ITS1-ITS2) along with 5.8S region from individual member were amplified by polymerase chain reaction (PCR) using universal primers and were sequenced. Sequences were uploaded in NCBI genbank, and were aligned and phylogenetic tree was constructed by neighbor joining method using online tools. ITS length and GC% was calculated using bioinformatics tool. (http://www.bitools.org). ITS length ranges from 524 to 638 base pairs (bp) where as GC% ranges from 53.4 to 61.8 in various Acanthaceae and Euphorbiaceae members. 5.8 S gene was found to be highly conserved and its size ranged from 178 to 259 bp. This combined data set provides a highly resolved hypothesis of relationship. Our analysis provides considerable resolution of relationship within various genera of family Acanthaceae and Euphorbiaceae. So ITS region may be considered as a good marker to study evolutionary history and to resolve controversies if any, in plant systematic.
Acanthaceae, Euphorbiaceae, nrITS, Phylogenetic
Cite This Article
Vishwesh R. Potkar, Satish N. Pardeshi, Pratima S. Jadhav, Pattern of Diversity Using Molecular Marker (nrITS): An Example from Acanthaceae and Euphorbiaceae, International Journal for Pharmaceutical Research Scholars, 2013, 2(4), 507-511.