Induction of Type-II Diabetes by High Fructose Diet and Low Dose of Intraperitoneal Injection of Streptozotocin in Albino Rats
Chandan Kumar, Raj Kumar, Shamshun Nehar
High fructose diet and streptozotocin (STZ) have been widely used separately to induce type-I and II diabetes in animal models especially rats. The objective of this study is to induce experimental type-II diabetes mellitus by combination of both high fructose and streptozotocin in normal adult wistar rats. Blood and urine glucose level, ketone and blood glucose level during oral glucose tolerance test (OGTT) were found much higher in treated diabetic group animals except plasma insulin in comparison to normal control group rats. Histopathology of pancreas of normal rats showed normal cellular structure of islets of langerhans whereas treated diabetic group rats showed degeneration, necrosis and extensive damage and depleted islets of langerhans. From the present study, it is concluded that, in albino rats, type-II diabetes can be induced by feeding fructose (21%) for 4 weeks followed by a single intra-peritoneal injection of STZ (40mg/kg body weight).
Diabetes Mellitus, Fructose, Insulin, Glucose, Streptozotocin, OGTT, Albino Rats
Cite This Article
Kumar, C., Kumar, R., & Nehar, S. (2014). Induction of Type-II Diabetes by High Fructose Diet and Low Dose of Intraperitoneal Injection of Streptozotocin in Albino Rats. International Journal for Pharmaceutical Research Scholars, 3(1), 196-202.