Identification of Total Phenolics, Total Flavonoids and Antioxidant Potential in the Leaf Extracts of Launaea procumbens Roxb

Identification of Total Phenolics, Total Flavonoids and Antioxidant Potential in the Leaf Extracts of Launaea procumbens Roxb


Mishra GJ, Rana JS, Gadhvi R, Reddy MN


Abstract

The present study is based on the aim to determine the possible use of plant Launaea procumbens in the field of therapeutics for oxidative stress and cancer studies. In the recent years, a trend for studies on oxidative stress has been remarkably raised. Currently used, synthetic antioxidants possess several side effects and also are suspected to have been promoting negative health effects. Therefore restrictions have been placed on their application, and curiosity is generated to substitute them with naturally occurring antioxidants. Hence, in the present study leaves of the plant Launaea procumbens were evaluated for the total phenolics, total flavonoids and antioxidant potential by DPPH radical scavenging assay. In vitro experimental assays on plant extract viz., Folin – Ciocalteus colorimetric method, Aluminum Chloride colorimetric method and DPPH radical scavenging assay were used with analysis being done on Graph-pad Prism Software for biological data. The concentration of total phenolics in test sample is calculated from the standard graph of quercetin and found to be 345 mg quercetin equivalent/gm of fresh mass, while the total flavonoids was calculated to be as 11.56 mg quercetin equivalent/gm of fresh mass. The antioxidant potential of the plant was calculated by % inhibition of the DPPH and found to be 200 µg/ml for >90 % inhibition with the IC50 value of 147.1. The results from the present studies revealed that the plant Launaea procumbens show marked scavenging potential with appreciable amount of total phenolics and total flavonoids.


Keywords

Total phenolics, Total flavonoids, Antioxidant potential, DPPH radical scavenging, Quercetin standard, Launaea procumbens


Cite This Article

Mishra, G. J., Rana, J. S., Gadhvi, R., & Reddy, M. N. (2014). Identification of Total Phenolics, Total Flavonoids and Antioxidant Potential in the Leaf Extracts of Launaea procumbens Roxb. International Journal for Pharmaceutical Research Scholars, 3(1), 374-381.

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