Phytochemical Analysis and In vitro Antioxidant Activity of Ochna obtusata

Phytochemical Analysis and In vitro Antioxidant Activity of Ochna obtusata


V Ravi Kumar*, T Shivaraj Gouda, Sreelakshmi S, Rajasekar


Abstract

The present study was carried out to evaluate the phytochemical constituents and in vitro antioxidant potential of Ochna obtusata. Qualitative phytochemical analysis was performed using extracts of five solvents methanol, ethanol, petroleum ether, chloroform and water to check for the presence of phytochemical constituents. Among the five extracts obtained, methanolic extract possessed to have the maximum phytochemical constituents comparatively and therefore used for further study. The antioxidant activity of methanolic extracts of Ochna obtusata was evaluated by various antioxidant assays which include DPPH assay, nitric oxide assay, reducing power assay and H2O2 assay. In all the tested methods Ochna obtusata has been found to possess the antioxidant activity in a dose-dependent manner. The present study revealed that Ochna obtusata is very rich in Phytochemicals and a good source of natural antioxidants.


Keywords

Ochna Obtusata, Anti-Oxidant, Free Radical Scavenging, DPPH Assay


Cite This Article

V Ravi Kumar, T Shivaraj Gouda, Sreelakshmi S, & Rajasekar. (2014). Phytochemical Analysis and In vitro Antioxidant Activity of Ochna obtusata. International Journal for Pharmaceutical Research Scholars, 3(4), 211-216.

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