In Vitro Anti-Cancer Activity of Quercetin and Kaempferol against Human Epithelial Malignant Melanoma Cells (A375)

In Vitro Anti-Cancer Activity of Quercetin and Kaempferol against Human Epithelial Malignant Melanoma Cells (A375)


Thoranavavi Kuppuswamy Gopal, Duraipandiyan Chamundeeswari*, Sekar Sathiya, Chidambaram Saravana Babu


Abstract

In the present study, in vitro anti-cancer activity of two flavonoids, quercetin and Kaempferol, was studied against human epithelial malignant melanoma cells (A375). Both the compounds were subjected to cytotoxicity assay. Quercetin and Kaempferol were investigated for their effects on apoptotic gene such as caspase3, bax, p53 and anti-apoptotic gene Bcl2 expression in A375 Cell lines by reverse transcriptase Polymerase Chain Reaction. MTT assays reveals that the IC50 value of quercetin and Kaempferol was found to be 1.54 and 12.05 ng/ml in the tested condition. Both the flavonoids down-regulated caspase3, bax, p53 and up-regulated Bcl2 expression. Results of the present study reveals that both the compounds may be interesting candidates for further studies on the molecular mechanism of action in skin carcinoma. 


Keywords

A375 Cell Lines, Quercetin, Kaempferol, Anti-Cancer, Apoptosis, mRNA Expression


Cite This Article

Thoranavavi Kuppuswamy Gopal, Duraipandiyan Chamundeeswari, Sekar Sathiya, & Chidambaram Saravana Babu. (2015). In Vitro Anti-Cancer Activity of Quercetin and Kaempferol against Human Epithelial Malignant Melanoma Cells (A375), International Journal for Pharmaceutical Research Scholars, 4(1), 157-162.

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