Validated Stability Indicating RP-HPLC Method for the Determination of Silodosin in Pharmaceutical Dosage Form

Validated Stability Indicating RP-HPLC Method for the Determination of Silodosin in Pharmaceutical Dosage Form


Harischandran S, Shankar Iyer R, Raju R, Shibi A, Sayana PS


Abstract

A simple, rapid and economic stability indicating high performance liquid chromatography method was developed for the determination of Silodosin in pharmaceutical dosage form. The chromatographic system comprised of a reverse phase Phenomenex C 18, 5µ Silica (250×4mm) column maintained at 25°C with mobile phase consisting of a mixture of methanol-water-acetonitrile-glacial acetic acid (60:27:10:3 % v/v) at pH 3.2 ± 0.1 with a flow rate of 1 ml/min, determined at 270 nm. The method was linear in the range of 10-100µg/ml. The results were validated according to ICH guidelines. The method could effectively separate the drug from its degradation products.


Keywords

Silodosin, HPLC, Stability indicating, Validation, Degradation.


Cite This Article

Harischandran S, Shankar Iyer R, Raju R, Shibi A, Sayana PS, Validated Stability Indicating RP-HPLC Method for the Determination of Silodosin in Pharmaceutical Dosage Form, International Journal for Pharmaceutical Research Scholars, 2012, 1(4), 141-145.

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