Simultaneous HPLC Determination of Ketoprofen and Famotidine in Laboratory Mixture
Boyka Tsvetkova*, Plamen Peikov, Ivelina Asenova
A simple, specific, precise and accurate reversed phase liquid chromatographic (RP-LC) method has been proposed for the simultaneous determination of ketoprofen and famotidine in laboratory mixtures. The chromatographic separation was performed on a LiChrosorb C18, 125 mm x 4.6 mm, 5 μm column at a detector wavelength of 230 nm and a flow rate of 1.5 ml/min. The mobile phase was composed of phosphate buffer (pH adjudted to 7.4 with ortho-phosphoric acid) and acetonitrile (20:80 v/v). The retention times of ketoprofen and famotidine were found to be 3.05 and 6.96 min, respectively. The method was validated for the parameters like specificity, linearity, precision, accuracy, ruggedness, limit of quantitation and limit of detection. The calibration curves were linear in the concentration range of 25.00-200.0 μg/ml for ketoprofen and 5.00-40.00 μg/ml for famotidine. The % recovery for both drugs was in the range between 98.33% and 99.85% with RSD values not greater than 2.55. The presented method for the simultaneous determination of ketoprofen and famotidine in synthetic mixture is specific, rapid and simple with good sensitivity.
Ketoprofen, Famotidine, HPLC, Validation, Laboratory Mixture, Quality Control
Cite This Article
Boyka Tsvetkova, Plamen Peikov, & Ivelina Asenova. (2014). Simultaneous HPLC Determination of Ketoprofen and Famotidine in Laboratory Mixture. International Journal for Pharmaceutical Research Scholars, 3(3), 347-352.