A Rapid High Performance Liquid Chromatography Method for Simultaneous Quantification of Praziquantel, Ivermectin and Abamectin from Veterinary Formulations: Development, Validation and Application

Author(s)

Phatak, H.M., Vaidya, V.V., Phatak, M.S., Rajeghadge, D.

Author's Affiliation

Abstract

A simple, sensitive, accurate and robust analytical method was developed for separation and quantification of Praziquantel, Ivermectin and Abamectin from pharmaceutical preparations. The chromatographic separation was achieved on a C8 column (Waters Luna C8, 250×4.6 mm, 5 µm) with a short runtime of 11 minutes. The mobile phase used was 65 parts of acetonitrile mixed with 20 parts of methanol and 15 parts distilled water. The flow rate was set to 1.5 ml/min while column oven was maintained at 30 ºC. The UV detector was set at 244 nm. The method was validated according to the regulatory guidelines with respect to specificity, precision, accuracy, linearity and robustness.  The working concentration for the assay method was 750 µg/ml for Praziquantel, 100 µg/ml for Ivermectin and 60 µg/ml Abamectin. Working range was selected based on the label claim of the oral paste formulations and keeping the final concentration of Praziquantel constant for the two formulations containing Ivermectin and Abamectin. Linearity range for the assay method was proven in the range of 375 to 1125 µg/ml for Praziquantel, 50 to 150 µg/ml Ivermectin and 40-80 µg/ml for Abamectin. The accuracy was between 98.0 to 102.0%. The precision was assessed by calculating %CV of six different assay preparations in intra-day. Intermediate precision was assessed as inter-day precision by calculating cumulative %CV of total twelve assay preparations. The cumulative %CV of twelve assay results was less than 2.0% for Praziquantel, Ivermectin and Abamectin. The method was found to be specific, finally, the method was demonstrated to be robust, resistant to small variations of chromatographic variables for oral paste formulations containing Praziquantel, Ivermectin and Abamectin.

Keywords

Praziquantel, Ivermectin, Abamectin, HPLC-UV, Simultaneous Quantification, Formulation Analysis

Cite This Article

Phatak, H.M., Vaidya, V.V., Phatak, M.S., Rajeghadge, D. (2016). A Rapid High Performance Liquid Chromatography Method for Simultaneous Quantification of Praziquantel, Ivermectin and Abamectin from Veterinary Formulations: Development, Validation and Application, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(1), 57-65.