A Sensitive RP-HPLC Method Development and Validation for the Simultaneous Estimation of Losartan Potassium and Hydrochlorothiazide

Author(s)

Ganipisetty, L. A., Dachinamoorthy, D., Rao, J. V. L. N.

Author's Affiliation

Abstract

A simple, accurate, sensitive and validated RP-HPLC method for simultaneous determination of Losartan and hydrochlorothiazide in combined tablet dosage form has been developed. Separation carried out on RP-HPLC system equipped with Inertsil ODS -3V Column (150 × 4.6 mm i.d., 5µm particle size) using mobile phase of Acetonitrile and phosphate buffer adjusted pH to 2 with orthophosphoric acid at a flow rate of 1 mL/min in the Gradient program with run time 25 minutes and detection using UV/VIS detector was carried out at 226 nm. Results were linear in the range of 20–150 μg/mL and 5-40 μg/mL for losartan and hydrochlorothiazide respectively. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.

Keywords

RP-HPLC, Losartan, Hydrochlorothiazide, Tablet Dosage Form

Cite This Article

Ganipisetty, L. A., Dachinamoorthy, D., Rao, J. V. L. N. (2014). A Sensitive RP-HPLC Method Development and Validation for the Simultaneous Estimation of Losartan Potassium and Hydrochlorothiazide. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 408-416.