A Validated RP-HPLC Method for Estimation of Rifaximin in its Bulk and Pharmaceutical Dosage Forms

Author(s)

Salla, D.R., Dhanthala, V.

Author's Affiliation

Abstract

An attempt has been made to develop and validate a simple, rapid, linear, precise, reproducible and economical method for Rifaximin in its bulk and pharmaceutical dosage forms by using Reverse Phase-HPLC method. The method was developed by using Reverse Phase-HPLC, the maximum wavelength was found to be at 276 nm. The linear regression coefficient was not more than 0.999. The estimation of Rifaximin was done by Reverse Phase-HPLC. The mobile phase optimised which consists of Acetonitrile: Triethylamine (TEA) of pH-4.6 mixed in a ratio of 70:30%v/v. A Phenomenex® Luna-C18 column (4.6×150cm, 5µm, Maker: waters) or equivalent chemically bonded porous silica particles as the stationary phase. The results obtained on the validation of parameters met the ICH and USP requirements. It inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with a high degree of accuracy and precision.

Keywords

Rifaximin, Acetonitrile, Triethylamine, Phenomenex® Luna-C18, Reverse Phase-HPLC, Orthophos-phoric acid (OPA)

Cite This Article

Salla, D. R., Dhanthala, V. (2017). A Validated RP-HPLC Method for Estimation of Rifaximin in its Bulk and Pharmaceutical Dosage Forms, International Journal for Pharmaceutical Research Scholars (IJPRS), 6(1), 11-20.