Analytical Method Development and Validation of Lamotrigine by High Performance Liquid Chromatography

Author(s)

Murthy, A. R., Babu, K. R., Vekariya, N.A.

Author's Affiliation

Abstract

In the present work, a rapid and sensitive RP-HPLC Method with UV detection (270 nm) for routine analysis of Lamotrigine in Bulk and in Pharmaceutical formulation was developed. Chromatography was performed with mobile phase containing a mixture of methanol and Phosphate buffer (70:30 v/v) with flow rate 1.1 ml min^-l. In the range of 20-100 μg/ml, the linearity of Lamotrigine shows a correlation co-efficient of 0.9998. Recovery of drug was found to be good (99-102%). Method was found to be reproducible with relative standard deviation (RSD) values less than 2% for intra and inter- day precision. The proposed method was validated as per the standard guidelines.

Keywords

Lamotrigine, bulk, formulation, HPLC

Cite This Article

Murthy, A. R., K. Babu, K. R., & Vekariya, N.A. (2014). Analytical Method Development and Validation of Lamotrigine by High Performance Liquid Chromatography. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(3), 155-161.