Antioxidant and Gastro-protective Activity of Ethanolic Rind Extract of Manihot Esculenta Crantz

Author(s)

Siyumbwa, S., Nomishan, N., Benny, Okechukwu, P. N.

Author's Affiliation

Abstract

Manihot Esculenta Crantz or better known as cassava  has been widely used to cure headache, hypertension, conjunctivis and cancer. In this study, we investigated the antioxidant and gastro-protective effect of ethanol rind extract of Manihotesculenta Crantz (Cassava) on ethanol and stress induced ulcer. The rind was dried in an oven and pure enthanol was used to extract the crude and simple biochemical and colorimetric assays were used to study the its antioxidant activity. Gastric ulcer were induced in the rat using absolute ethanol and stress. Oral administration of 100, 250 and 500mg/kg of the extract showed gatro-protective of 36.3%, 54.6% and 63.7% respectively for stress induced ulcer and 42.2%, 49.1% and 70.2% respectively for ethanol induced ucler. The pH of the stomach in both ulcer models were  increased  from 2.84-7.13 after oral administration of the extracts. It showed total Antioxidant Capacity (TAC) of  36.13 ± 5.76 µg AAE/mg DW, total Proanthocyanidin (TPR) 25.9±1.41 µg CATE/mg DW and Total Phenolic Content (TPC) 28.9µg GAE/mg DW. The concentration needed for 50% scavenging activity of DPPH, ABTS, SO, and NO was found to be 32.22, 0.161, 107.3 and 0.372 mg/ml, respectively and reducing power of 1.126. Cassava rind extract showed gastroprotective effect against ethanol and stress induced ulcer and this effect maybe because of its antioxidant property.

Keywords

Cassava, Peels, Antioxidant activity, Total phenolic content, Gastro-protective

Cite This Article

Siyumbwa, S., Nomishan, N., Benny, & Okechukwu, P. N. (2014). Antioxidant and Gastro-protective Activity of Ethanolic Rind Extract of Manihot Esculenta Crantz. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 798-808.