Assay of Olanzapine in Pharmaceutical Formulations by Visible Spectrophotometry using Cadmium (II)

Author(s)

Reddy, K. N., Giri, A., Saritha, B., Reddy, T. S.

Author's Affiliation

Abstract

Cadmium (II) reacts with olanzapine in the pH range 8.0-12.0 forming a yellow colored complex solution which has sufficient   absorbance at 420 nm. Studies were carried at pH 10.0. The colour intensity attains a maximum value after 30 minutes at 60^o C. The straight line relation between absorbance and amount of olanzapine obeys the equation A = 0.0141 C - 0.00004. The linear plot indicates that Beer’s law is obeyed in the range 4.0-40.0 µg/ml of olanzapine. The molar absorptivity and sandell’s sensitivity are 4.375×10³ l mol^-1 cm^-1 and 0.0714 µg cm^-2 respectively. The standard deviation of the method for ten determinations of 25 µg/ml olanzapine is 0.0016. The correlation coefficient (g) of the experimental data of the calibration plot is 0.9999. The effect of various excipients was studied. The composition of the complex is established as 1:2 [Cd (II): olanzapine]. The stability constant of the complex is 9.822×10¹². The developed method was validated according to ICH guidelines and was found to be accurate and precise. The validation parameters such as, linearity, accuracy, precision, LOD, LOQ and ruggedness were studied. The proposed method   for the quantitative estimation of olanzapine is accurate, precise, highly sensitive and selective, for the estimation of olanzapine in its pharmaceutical formulations. Hence the proposed method is successfully applied for the determination of olanzapine in pharmaceutical formulation.

Keywords

Olanzapine, Cd (II), Visible Spectrophotometry, Method Validation

Cite This Article

Reddy, K. N., Giri, A., Saritha, B., Reddy, T. S. (2014). Assay of Olanzapine in Pharmaceutical Formulations by Visible Spectrophotometry using Cadmium (II). International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 417-424.