Development and Evaluation of In-Vitro Antioxidant Potential and In-Vivo Anti-Ageing Activity of Polyherbal Formulation

Author(s)

Shinde, V.M., Zingade, S., Kamble, R.N., Mugade, M.V., Nangare, P.S.

Author's Affiliation

Abstract

Aging is indicated by a slow, gradual, structural and functional decline transformation that occurs at various levels of cells, tissues and organs. In human body, oxidative stress play major role in ageing process. Now a day, the free radical theory of ageing can help to understand the process of ageing and search for the effective anti ageing agents. Previous literature review of research has indicated that many of the traditional plants possess potent anti-ageing activity. Present study focuses on different theories of ageing and anti-ageing effects of polyherbal formulation (PHF) of Emblica officinalis, Curcuma longa, Tribulus terrestris and Asparagus racemosus. Moreover, the phytochemical characterization and antioxidant potential of the extract was also measured by determining total phenolic contents, DPPH (2,2-diphenyl-β-picrylhydrazyl), reducing power assay which are estimated in in-vitro study. In-vivo anti-ageing activity performed by using D-galactose induced ageing model. Biochemical investigation was done for lipid peroxidation, lipofuscinogensis and total protein. The present study demonstrated that PHF have significant anti ageing capacity, safety and potential to demolish the oxidative stress in body.

Keywords

Polyherbal, Ageing, Oxidative Stress, Antioxidant, DPPH

Cite This Article

Shinde, V.M., Zingade, S., Kamble, R.N., Mugade, M.V., Nangare, P.S. (2016). Development and Evaluation of In-Vitro Antioxidant Potential and In-Vivo Anti-Ageing Activity of Polyherbal Formulation, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(2), 116-127.