Development and Validation of a Rapid High Performance Liquid Chromatography Method for Simultaneous Quantification of Ornidazole and Miconazole from Cream Formulations

Author(s)

Phatak, M. S., Vaidya, V. V., Phatak, H. M.

Author's Affiliation

Abstract

High temperature and humidity typically describe the tropical regions in which Indian sub-continent lies. These conditions are the breeding grounds required for opportunistic fungal and other infections. The common treatment for fungal infections are azole antibiotics. However, combination of two drugs provides better effect. For an analytical chemist this is a challenge as many of the times the active ingredients (analytes) have distinct chemical and physical properties leading difficulty in developing a single method which can be used for the quantitation of these drugs. In the current research a rapid analytical method employing HPLC has been developed and validated for simultaneous quantification of the active ingredients Ornidazole and Miconazole from the cream formulation. The analytes were extracted from cream base and filtered. An ODS column enabled chromatographic is used for the separation of the analytes. The method involves simple isocratic chromatography and UV detection. Validation of the method showed response was a linear function of concentration in the range 50-150 µg mL⁻¹ for both Ornidazole and Miconazole. The method was suitably validated and was found to be precise and robust, with recoveries for both the analytes being consistent and complete. The method has been successfully applied for the analysis of samples from marketed cream formulations.

Keywords

Ornidazole, Miconazole, HPLC, Formulation Analysis

Cite This Article

Phatak, M. S., Vaidya, V. V., & Phatak, H. M. (2014). Development and Validation of a Rapid High Performance Liquid Chromatography Method for Simultaneous Quantification of Ornidazole and Miconazole frrom Cream Formulations. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(4), 191-197.