Development and Validation of a RP-HPLC Method for the Simultaneous Estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in Tablet Dosage Forms

Author(s)

Kantariya, B., Agola, A., Roshani, H., Ghetia, U., Shivam, S. S.

Author's Affiliation

Abstract

An accurate, precise and reproducible high performance liquid chromatographic method was developed for simultaneous estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in tablet dosage forms. Chromatographic separation of the drugs were achieved on a Phenomenax C18 column (150 x 4.6 mm; 5μ) using a mobile phase consisting of ortho-Phosphoric acid 0.1% and Acetonitrile pH 3.5 (25:75, %v/v) at a flow rate of 0.5 ml/min. The drugs elute were monitored at 218 nm. The retention time obtained for the Ranitidine Hydrochloride was 4.32 min and for the Dicyclomine Hydrochloride was 5.96 min. The calibration curves were linear over the range of 5-25 μg/ml and 50-250 μg/ml for Ranitidine Hydrochloride and Dicyclomine Hydrochloride respectively. The performance of the method was validated according to ICH guidelines. The method could be applied for determination of in its tablet dosage forms without any interference from excipients or endogenous substances. The proposed method is suitable for routine quality control analysis.

Keywords

High Performance liquid Chromatography, Ranitidine Hydrochloride (RANTD), Dicyclomine Hydrochloride (DICY)

Cite This Article

Kantariya, B., Agola, A., Roshani, H., Ghetia, U., & Shivam, S. S. (2013). Development and Validation of a RP-HPLC Method for the Simultaneous Estimation of Ranitidine Hydrochloride and Dicyclomine Hydrochloride in Tablet Dosage Forms. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(2), 258-267.