Development and Validation of Difference Spectrometric Method for the Estimation of Garenoxacin mesylate in Bulk and Pharmaceutical Formulation

Author(s)

Sakariya, S.V., Mardia, R.B., Chauhan, S.P., Suhagia, B.N.

Author's Affiliation

Abstract

A simple, specific and rapid difference spectroscopic method has been developed for the estimation of Garenoxacin mesylate in bulk and Pharmaceutical formulation. The proposed method was carried out by measuring the difference absorbance of Garenoxacin mesylate in two different conditions containing three different forms of drug generated by neutral (solvent), acidic (solvent) and basic (solvent) medium. The measurements of difference absorbance were carried out at 347nm and 348nm for two different conditions. The calibration curves were linear in the concentration range of 10-50µg/ml. The proposed method was validated as per ICH validation guideline Q₂ (R₁) for accuracy, robustness, LOD, LOQ etc. The method was found to be accurate, precise, robust and sensitive hence it can be applied in routine analysis of Garenoxacin mesylate in bulk and Pharmaceutical formulation in its quality control.

Keywords

Difference Spectrometry, Garenoxacin Mesylate, 0.1N HCl, 0.1N NaOH, Distilled Water

Cite This Article

Sakariya, S.V., Mardia, R.B., Chauhan, S.P., & Suhagia, B.N. (2015). Development and Validation of Difference Spectrometric Method for the Estimation of Garenoxacin mesylate in Bulk and Pharmaceutical Formulation, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 355-360.