Development and Validation of High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Etamsylate and Tranexamic Acid in Tablet Dosage Form

Author(s)

Naik, R.Y., Dedania, Z., VijayendraSwamy, S.M., Chorawala, H.

Author's Affiliation

Abstract

A simple, novel, rapid, precise, accurate, specific and cost effective High performance thin layer chromatographic method has been developed and validated for simultaneous determination of Etamsylate and Tranexamic Acid in combined tablet dosage form. The stationary phase used was precoated silica gel 60F₂₅₄ plate.  The mobile phase used was the mixture of water: acetone: methanol (3:4:3v/v/v). The detection of spots was carried out densitometrically using a UV detector at 230nm in absorbance mode. Developed method was validated according to the International Conference on Harmonization (ICH) guidelines. Calibration curve was found to be linear between 2000 to 12000 ng/spot for both drug with correlation coefficient 0.9993 and 0.9988 for Etamsylate and Tranexamic acid respectively. Accuracy in terms of % recovery was found to be 98.95-99.50 and 98.41-99.54 for Etamsylate and Tranexamic acid respectively. Limit of detection for Etamsylate and Tranexamic was found to be 338.60 ng/spot and 440.07ng/spot respectively. Limit of quantitation for Etamsylate and Tranexamic was found to be 1026.07 ng/spot and 1333.55 ng/spot respectively. Thus the proposed method can be successfully applied for simultaneous determination of Tranexamic Acid and Etamsylate in combined tablet dosage form.

Keywords

Etamsylate, Tranexamic acid, High performance thin layer chromatography, Validation

Cite This Article

Naik, R.Y., Dedania, Z., VijayendraSwamy, S.M., Chorawala, H. (2015). Development and Validation of High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Etamsylate and Tranexamic Acid in Tablet Dosage Form. International Journal for Pharmaceutical Research Scholars (IJPRS), 4(2), 1-9.