Development and Validation of HPTLC Method for Simultaneous Estimation of Esomeprazole Magnesium and Aspirin in Bulk and Synthetic Mixture

Author(s)

Patel, B., Parmar, S., Doshi, J., Captain, A. D.

Author's Affiliation

Abstract

A simple, accurate, and precise HPTLC method has been developed and validated for the simultaneous estimation of Esomeprazole magnesium (ESO) and Aspirin (ASP) from bulk drug and Synthetic mixture. The method employed TLC aluminum plates precoated with silica gel 60 GF ₂₅₄ as the stationary phase. The solvent system comprised Acetate: Toluene: Glacial acetic acid (4.5:0.5:0.05 v/v/v). This system was found to give good result for both the drugs (Rf value: of ESO 0.22cm and ASP 0.78cm). Spectrodensitometric scanning-integration was performed at a wavelength of 239nm.The calibration curve was found to be linear within the concentration range of 200ng/spot to 800ng/spot for both the drugs. The regression data for calibration curve shows good linear relationship with r² = 0.9981 and 0.9990 for ASP and ESO respectively. The method was validated in accordance with the requirements of ICH guidelines. The method was successfully applied for determination of drug in bulk and synthetic mixture. Thus, the proposed method can be used successfully for routine analysis of ESO and ASP from bulk and Synthetic mixture.

Keywords

Validation, HPTLC, Esomeprazole Magnesium, Aspirin

Cite This Article

Patel, B., Parmar, S., Doshi, J., & Captain, A. D. (2014). Development and Validation of HPTLC Method for Simultaneous Estimation of Esomeprazole Magnesium and Aspirin in Bulk and Synthetic Mixture. International Journal for Pharmaceutical Research Scholars, 3(1), 236-242.