Effect of Ruta Graveolens Extract on Histopathologic Changes in Mice Livers

Author(s)

Nabaei, M., Mesbah, A. R., Ghavami, H., Saeidinia, A., Keihanian, F.

Author's Affiliation

Abstract

Ruta had been used in different nation traditional medicine specifically in Iranian traditional medicine and marked properties had been mentioned for it. In old references using Ruta in some cases caused failure in vital organs of body such as liver and kidney and also mortality. This study had been performed to survey effects of Ruta graveolens extract on histopathlogic changes in mice livers. This is an experimental study which made on 30 NMRI mice. Mice were divided in 6 quintuple groups. Groups 1 to 4 injected by the doses of 200,300,400 and 500 mg/kg of hydro-alcoholic extracts respectively. Solvent of extract injected in group 5 mice and group 6 mice were intact. The injection was performed intraperitoneal and three times in a week. In the 7th day mice were anesthetized deeply then liver tissues were extracted and after H&E coloring analyzed pathologically. Different pathologic factors were studied in liver of mice. Only common positive factor in all groups were a little inflammation around portal space in comparison with control group. Pathologic results show that inflammation and cell infiltration was increased dose dependent. Any fatty change, fibrosis and evidences of cholestasis were not seen and there was no change in control group subjects. It seems that different doses of hydro-alcoholic extract of Ruta graveolens had no toxic effect on histopathology of liver except in high dose. In order to the results high doses (500 mg/kg) of herb can lead to histologic hepatotoxicity and careful care for using this herb should be done in long term with using certain non-toxic doses and avoiding self-medication.

Keywords

Ruta, Liver Toxicity, Hydro-Alcoholic Extract

Cite This Article

Nabaei, M., Mesbah, A. R., Ghavami, H., Saeidinia, A., Keihanian, F., Roudbarchi, A., Saeidinia, F., & Morovati, H. (2014). Effect of Ruta Graveolens Extract on Histopathologic Changes in Mice Livers. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 675-680.