High-Performance Liquid Chromatography: An Overview

Author(s)

Tandia, N., Singh, S. K., Kumar, N., Singh, S.

Author's Affiliation

Abstract

Chromatography is considered extremely powerful separation technique for variety of samples. The common feature of all these techniques is that the different components in a sample mixture are distributed between two phases, one of which remains stationary while the other the mobile phase, runs through the interstices or over the surface of the fixed phase. The movement of the mobile phase results in differential migration of the sample components. High performance thin layer chromatography is an invaluable quality assessment tool for the evaluation of botanical materials and the equally suitable for qualitative and quantitative  analysis of biological samples which are of particular interest to biologists, biochemists, hematologists, immunologists, medical diagnosticians, and molecular biologists. It allows for the analysis of a broad number of compounds efficiently and cost effectively. Over three decades of development in HPLC have given us a well understood separation tool capable of very reliable results. Refinements in HPLC column technology have lead to high resolution separations, fast analysis, and high sensitivity. However, to obtain all the benefits that HPLC can offer, careful attention must be paid to that part of the liquid flow system that carries the sample.

Keywords

HPLC, Sample, Chromatography, Instrumentation technique

Cite This Article

Tandia, N., Singh, S. K., Kumar, N., Singh, S. (2013). High-Performance Liquid Chromatography: An Overview. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(4), 1-13.