Histopathological Changes in Three Variations of Wistar Rat Adjuvant-Induced Arthritis Model

Author(s)

Laste, G., Souza, I. C. C., Santos, V. S. D., Caumo, W., Torres, I. L. S.

Author's Affiliation

Abstract

This study aims to describe histopathological changes observed in three Wistar rat variations of adjuvant-induced arthritis model inducing mono arthritis and poly arthritis. Complete Freund’s adjuvant (CFA) was injected in variation I: 200 μL of emulsion injected into the right footpad; CFA-induced arthritis variation II: 100 μL of CFA injected into the right footpad and two injections of 100 μL into the tail; CFA-induced arthritis variation III: 300 μL of CFA injected into the right footpad. All animals presented joint damage, revascularization, and synovial proliferation. Analysis of histological scores on the 15th day showed that variation I had the highest scores for synovial inflammation (3.0±0) and subcutaneous inflammation (3.0±0), and variation III presented the highest scores for cartilage (2.3±1.1) and bone erosion (2.3±1.1). On the 21st day, once again variation I showed the highest scores for synovial (2.3±0.6) and subcutaneous inflammation (3.0±0.6), despite a decrease in synovial inflammation scores on the 21st day (3.0 to 2.3). Cartilage erosion was more frequent in variation II (2.0±1.1), and bone erosion was similar in all models. Our findings suggest that arthritis was successfully induced in all three variations of Wistar rat arthritis model, with variable severity in terms of histological findings and clinical manifestations. The most homogenous response was obtained with the use of an emulsion to induce arthritis, however with lesser severe manifestation.

Keywords

Adjuvant-Induced Arthritis, Wistar Rats, Histopathological Abnormalities

Cite This Article

Laste, G., Souza, I. C. C., Santos, V. S. D., Caumo, W., & Torres, I. L. S. Histopathological Changes in Three Variations of Wistar Rat Adjuvant-Induced Arthritis Model. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 780-790.