HPLC Method for Quantification of Berberine in Wild and Micropropagated Tinospora cordifolia – An Important Ayurvedic Medicinal Plant

Author(s)

Sivakumar, V., Sadiq, A. M., Rajan, M. S. D., Jayanthi, M.

Author's Affiliation

Abstract

The present study was attempt to established for rapid clonal propagation of valuable medicinal plant, Tinospora cordifolia, through in vitro culture using nodal explants from selected plant. And a sensitive, simple and accurate HPLC method had been developed for quantification of  berberine, a isoquinoline alkaloid in dry stem of wild and micropropagated Tinospora cordifolia (wild) Miers. ex Hook.f. & Thoms. The chromatographic analysis was performed using pet- ether, methanol, aqueous, chloroform extracts of wild and micropropagated plant stem methanol extract, on using a solvent system, comprising of Acetonitrile: water 60:40 (V/V) as mobile phase at a flow rate of 0.5ml/min. And 265nm gives good separation of berberine at Rt 5.15min. The maximum of berberine content was observed in methanol extract of micropropagated plant stem when compared to wild test samples. The proposed HPLC method is rapid and accurate for quantitative monitoring of berberine in Tinospora cordifolia.

Keywords

Tinospora cordifolia, Micropropagation, Berberine, HPLC

Cite This Article

Sivakumar, V., Sadiq, A. M., Rajan, M. S. D., Jayanthi, M. (2014). HPLC Method for Quantification of Berberine in Wild and Micropropagated Tinospora cordifolia - An Important Ayurvedic Medicinal Plant. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 432-439.