In Silico Analysis of Antiviral Proteins in Clerodendrum Inerme

Author(s)

Sathya, R., Renuka, R., Bharathi, N.

Author's Affiliation

Abstract

Clerodendrum inerme is used in Indian and Chinese traditional medicine. It is reported to exhibit antiviral property against mammalian viruses. In this study, antiviral property of C. inerme against the economically important plant viruses such as, Cowpea mosaic virus (CoMV), Cowpea chlorotic mottle virus (CCMV), Cucumber mosaic virus (CuMV), Potato yellow mosaic virus (PYMV), Satellite tobacco mosaic virus (STMV) and Tobacco mosaic virus (TMV) is analyzed in silico using protein-protein interaction studies. The proteins in C. inerme, viz., CIP-29 and Crip-31 were found to exhibit antiviral property. Site directed mutagenesis of these two proteins using in silico methods was also studied. The analysis revealed that there is effective binding of the viral proteins and plant proteins with minimum binding energy. The study also revealed that the native plant proteins, CIP-29 and Crip-31are more effective to interact with viral proteins than the in silico mutated versions.

Keywords

Clerodendrum inerme, Antiviral property, In silico analysis, Site directed mutagenesis, ClusPro Server 2.0

Cite This Article

Sathya, R., Renuka, R., & Bharathi, N. (2014). In Silico Analysis of Antiviral Proteins in Clerodendrum Inerme. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 661-669.