Induction of Apoptotic Cell Death by Naringenin in DEN Induced Hepatocarcinogenesis in Rats

Author(s)

Georget, D. J., Subramaniam, J., Thiruvengadam, D.

Author's Affiliation

Abstract

Naringenin (4,5,7-trihydroxyflavone) (NGEN) a flavanoid belonging to the subclass of flavanol is believed to play a pivotal role in apoptosis. In this study we found that co-treatment of Naringenin (50mg/kg body weight) for 16 weeks to N-Nitrosodiethylamine (DEN) induced rats thereby promoting apoptosis and blocking the down regulation of the PI3k/ Akt pathway. A better understanding of its biology could lead to improved treatment options. Generally, the goal of cancer treatment is to abolish cell proliferation and to induce necrotic or apoptotic cell death. Different apoptotic signals converge to induce caspase cascade activation. Caspase 3 and Caspase-9 is necessary for effective apoptotic cell death. Bcl-2 protein family regulates apoptosis. The Bcl-2 protein itself is a product of a proto-oncogene and has an antiapoptotic action. Analysis of morphological changes of hepatocytes and protein expressions of various apoptotic related markers, such as caspase-9, caspase-3, Bcl-2 Bax and Akt disclosed that the apoptotic activities of NGEN may be mediated through eliciting apoptotic mechanism in DEN-induced HCC condition.

Keywords

Naringenin, N-Nitrosodiethylamine, Apoptosis, Caspase 9, Caspase 3, Akt

Cite This Article

Georget, D. J., Subramaniam, J., & Thiruvengadam, D. (2013). Induction of Apoptotic Cell Death by Naringenin in DEN Induced Hepatocarcinogenesis in Rats. International Journal for Pharmaceutical Research Scholars (IJPRS), 2(4), 558-564.