Isolation, Molecular Characterization and In-silico Analysis of Erwinia carotovora from Rotten Vegetables

Author(s)

Rayavarapu, K. A., Kaladhar, D. S. V. G. K., Vadlapudi, V.

Author's Affiliation

Abstract

Erwinia carotovora, a plant pathogen that causes soft-rot disease, dependent on the production and secretion of a complex arsenal of plant cell wall degrading enzymes. Biochemical characteristics of Erwinia carotovora with other bacterial species from spoiled Potatoes, carrots and tomatoes have been conducted to know biological parameters and pathogenicity relationships.  Gram staining, Isolation of DNA and Northern blotting methods has been conducted in the present experimentation. Gram staining results shows purple coloured rod shaped Gram +ve bacteria. Biochemical activity has also shown good results. The DNA isolate of bacteria such as Erwinia carotovora, Aeromonas hydrophila, Pseudomonas aeruginosa has observed bands at 12000 bp, Staphylococcus aureus has observed bands at 12,500 bp, and Xanthomonas citri has observed bands at 11,000 bp. In these five organisms, highest molecular weight is observed in Staphylococcus aureus and lowest molecular weight observed in Xanthomonas citri. The species contains translocation and biodegradable proteins related to other bacterial species. Erwinia carotovora that causes soft-rot diseases in plants like Potatoes, Carrots and Tomatoes containing convergence with other species such as Aeromonas hydrophila, Pseudomonas aeruginosa, and shows divergence with Xanthomonas citri and Staphylococcus aureus.

Keywords

Erwinia carotovora, Biochemical tests, Clinical isolation, Gram staining, DNA isolation, SDS PAGE

Cite This Article

Rayavarapu, K. A., Kaladhar, D. S. V. G. K., Vadlapudi, V. (2014). Isolation, Molecular Characterization and In-silico Analysis of Erwinia carotovora from Rotten Vegetables. International Journal for Pharmaceutical Research Scholars, 3(1), 864-871.