New RP-HPLC Method Development and Validation of Sulfapyridine in Pure and Tablet Dosage Forms

Author(s)

Tirumaleswara Rao, B., Madhavi, S., Sudheer, C.H.

Author's Affiliation

Abstract

The objective of this work was to develop a simple, selective, stability indicating RP-HPLC method for the determination of sulfapyridine in pure and in tablet dosage form. The proposed RP-HPLC method was validated in compliance with International Conference on Harmonization guidelines. The mobile phase consists a mixture of Acetonitrile: water: 1.0 % ortho phosphoric acid in the ratio of 70:27:3 v/v/v.  Column used ODS, C₁₈ RP- COLUMN (4.6mmx250mm) having 5µm particle size. The flow rate was 1.0 mL/min and detection was carried out by UV- visible spectrophotometer and was observed that the maximum absorbance (λmax) was obtained at 256nm, retention time 4.40mins. The proposed method has permitted the quantification of sulfapyridine over linearity in the range of 5-30µg/mL and its percentage recovery was found to be 99.99%. The proposed RP-HPLC method of sulfapyridine was also found to be robust and rugged as there was no significant change in the peak area, peak shape and retention time. On the basis of above facts it is concluded that “the developed RP-HPLC method was found to be easily applicable and is expected to be widely used for the routine QC analysis of sulfapyridine in the pharmaceutical industry”.

Keywords

Sulfapyridine, Method Development, Validation, RP-HPLC

Cite This Article

Tirumaleswara Rao, B., Madhavi, S., & Sudheer, C.H. (2016). New RP-HPLC Method Development and Validation of Sulfapyridine in Pure and Tablet Dosage Forms, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(1), 66-74.