Phytochemical Screening and Estimation of Total Phenolics and Total Flavonoid Content of Lagenaria Siceraria, Praecitrullus Fistulosus (50:50) Fruit and their Mixture

Author(s)

Tyagi, N., Madan, H., Pathak, S.

Author's Affiliation

Abstract

Cucurbitaceae family is major source of medicinal agents since ancient time. Various plants parts including fruits of this family have been established for their pharmacological potential. In the series of Cucurbitaceae plants, Praecitrullus fistulosus and Lagenaria siceraria is one of the excellent plants, gifted by the nature having composition of all the essential constituents that are required for normal and good human health. The aim of present study was to investigate the phyto-constituents present within the ethanolic extract of Praecitrullus fistulosus fruit and to estimate the total phenolic and total flavonoid contents. The amount of total phenols, were analyzed using a spectrophotometric technique, based on Folin-ciocalteau reagent. Gallic acid was used as standard compound and the total phenols were expressed as mg/g gallic acid equivalents (Standard curve equation:  Y = 0.008X + 0.003, R²= 0.998). The total phenolic content in ethanolic extract of Lagenaria siceraria, Praecitrullus fistulosus and their mixture was 31.42 mg/g, 25.71 mg/g and 27.71 mg/g respectively. Total flavonoid contents were estimated, using a spectrophotometric technique. The rutin was used as standard compound and the total flavonoids were expressed as mg/g rutin equivalents (Standard curve equation: Y = 0.006X + 0.001, R²= 0.998). The total flavonoid content in ethanolic extract of Lagenaria siceraria, Praecitrullus fistulosus and their mixture was 26.6 mg/g, 19.8 mg/g and 23.4 mg/g respectively. The maximum phenolic and flavonoid content were found in ethanolic extract of Lagenaria siceraria 31.42 and 26.6 mg/g respectively.

Keywords

Praecitrullus Fistulosus, Lagenaria Siceraria Phytoconstituents, Phenols, Flavonoids

Cite This Article

Tyagi, N., Madan, H., & Pathak, S. (2014). Phytochemical Screening and Estimation of Total Phenolics and Total Flavonoid Content of Lagenaria Siceraria, Praecitrullus Fistulosus (50:50) Fruit and their Mixture. International Journal for Pharmaceutical Research Scholars (IJPRS), 3(2), 882-890.