Regularization of Estrous Cycle Using Pergularia Daemia and Metformin in the PCOS Induced Rats

Author(s)

Nivetha, S., Poornima, R., Horne, I.A.

Author's Affiliation

Abstract

Polycystic ovary syndrome (PCOS) is most common heterogeneous, endocrinological and metabolic disorder affecting, reproductive aged women. Nearly 14.6% - 22.8% of PCOS women have the prevalence of menstrual dysfunction and irregularities ranging from amenorrhea to menorrhagia with classic peripubertal onset. In this study, the herbal plant Pergularia daemia is used treat PCOS by using female albino wistar rat as a model. The experimental animals were divided into four groups each group consisting of five rats. 1% of CMC (carboxy methyl cellulose) was received by control group (Group I) for 21 days. The remaining three groups were administrated with letrozole (1mg/kg dissolved in 2.0 ml/kg) orally for 21 days. The first letrozole treated group is considered to be Group II, which serves as a PCOS model. The second letrozole treated group is considered as Group III where (2mg/100g) of metformin is treated for 15 days and the final group (Group IV) was treated with methanolic leaf extract of Pergularia daemia (0.5 ml) for 7 days. The vaginal smear was collected every day to find changes in estrus cycle. The control group showed no changes in the estrous cycle whereas the PCOS induced group had an altered estrous cycle. The metformin and Pergularia daemia treated groups showed improvement but when compared to metformin, the Pergularia daemia treated group showed more regularity in the cycle. Thus the methanolic extract of Pergularia daemia has the potentiality to regain the estrous cycle in the PCOS induced rats.

Keywords

Pergularia Daemia, Albino Wistar Rat, Letrozole, Estrous Cycle, Metformin

Cite This Article

Nivetha, S., Poornima, R., Horne, I.A. (2016). Regularization of Estrous Cycle Using Pergularia Daemia and Metformin in the PCOS Induced Rats, International Journal for Pharmaceutical Research Scholars (IJPRS), 5(3), 99-103.