Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Pregabalin and Nortriptyline in Tablet

Author(s)

Mevada, N.A., Patel, B.R., Patel, J.G., Vegad, K.L., Patel, V.S.

Author's Affiliation

Abstract

Stability indicating a RP-HPLC method for simultaneous estimation of Nortriptyline Hydrochloride and Pregabalin in their Combined Dosage Form has been developed. A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Nortriptyline Hydrochloride and Pregabalin in their Combined Dosage Form has been developed. The separation was achieved by LC- 20 AT C₁₈ (250mm x 4.6 mm x 2.6 µm) column and Phosphate Buffer (pH 5.0): Methanol (70:30, V/V) as mobile phase, at a flow rate of 1ml/min. Detection was carried out at 210 nm. The retention time of Nortriptyline Hydrochloride and Pregabalin were found to be 3.203 min and 5.400 min, respectively. The method has been validated for linearity, accuracy, and precision. Linearity observed for Nortriptyline Hydrochloride 5-15 μg/ml and for Pregabalin 37.5-112.5 μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Nortriptyline Hydrochloride and Pregabalin in their Combined Dosage Form.

Keywords

Nortriptyline Hydrochloride, Pregabalin, Stability indicating RP-HPLC Method

Cite This Article

Mevada, N.A., Patel, B.R., Patel, J.G., Vegad, K.L., Patel, V.S. (2017). Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Pregabalin and Nortriptyline in Tablet.  6(2), 1-7.