Stability Indicating RP-HPLC Method Development and Validation of Simultaneous Estimation of Trandolapril and Verapamil Hydrochloride with Forced Degradation Studies in Bulk and Commercial Products

Author(s)

Ganipisetty, L. A., Dachinamoorthy, D., Rao, S.

Author's Affiliation

Abstract

A simple reproducible and efficient isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous estimation of Trandolapril and Verapamil. All the drugs were separated on an Inertsil ODS – 3V 150 x 4.6mm, 5µm. The mobile phase, optimized through an experimental design, was a 40:60 (v/v) mixture of acetonitrile and triethylamine buffer (pH 3.0 ), pumped at a flow rate of 1.3 ml/min. UV detection was performed at 216 nm. The retention time of Verapamil Hydrochloride and Trandolapril was found to be 1.51 min and 3.43 min respectively. The method was validated in the sample concentration ranges of 1.6 - 25µg/ml for Trandolapril and 95-1530µg/ml for Verapamil Hydrochloride, The method demonstrated to be robust, resisting to small deliberate changes in pH and flow rate of the mobile phase. The LOD values were 0.26 µg/ml and 10.3 µg/ml, while the LOQ values were 0.87 µg/ml and 31.1 µg/ml for Trandolapril and Verapamil Hydrochloride respectively. The recoveries for all three levels were above 99%.

Keywords

RP-HPLC, Tablet dosage form, Trandolapril, Verapamil

Cite This Article

Ganipisetty, L. A., Dachinamoorthy, D., & Rao, S. (2015). Stability Indicating RP-HPLC Method Development and Validation of Simultaneous Estimation of Trandolapril and Verapamil Hydrochloride with Forced Degradation Studies in Bulk and Commercial Products, International Journal for Pharmaceutical Research Scholars (IJPRS), 4(4), 1-9.