Zero Order and First Order Derivative Spectrophotometric Methods for Determination of Clonazepam in Pharmaceutical Dosage Form

Author(s)

Rivonker, N., Walke, T.V.

Author's Affiliation

Abstract

A simple, precise and economical spectrophotometric method has been developed for estimation of clonazepam in pharmaceutical dosage form using distilled water as a solvent. The quantitative determination of the drug was carried out using the zero order derivative values measured at 307nm and the first order derivative values measured at 330nm. The method was found to be linear and obeys Beer’s law in concentration range 2-22µg/ml with a coefficient of correlation values for zero and first order derivative method 0.9994 and 1.0 respectively. The percent mean recovery for zero and first order derivative was found to be 100.5% and 97.9% respectively. The mean percentage drug content for zero and first order derivative methods was found to be 99.75% and 102% respectively, and %RSD value was found to be less than 2 which shows the precision of the method. The limit of detection and limit of quantitation for zero order derivative was found to be 0.6739µg/ml and 2.042µg/ml respectively. The developed method was validated according to ICH guidelines and found to be accurate and precise. Thus the proposed method can be successfully applied for the determination of clonazepam in the pharmaceutical dosage form.

Keywords

Clonazepam, Zero order derivative spectrum, First order derivative spectrum, Validated

Cite This Article

Rivonker, N., Walke, T.V. (2018). Zero Order and First Order Derivative Spectrophotometric Methods for Determination of Clonazepam in Pharmaceutical Dosage Form. International Journal for Pharmaceutical Research Scholars (IJPRS), 7(1), 50-54.